Journal: bioRxiv
Article Title: Trophoblast ferroptosis restricts SARS-CoV-2 spread in the placenta
doi: 10.64898/2026.01.26.701742
Figure Lengend Snippet: (A) Viral titers of SARS-CoV-2 Delta variant in supernatants from JEG-3 cells infected over 48 hours with an MOI of 1. Titers were determined via plaque assay. (B-E) Protein expression of iron transport and storage proteins in SARS-CoV-2-infected JEG-3 cells at 6, 24, and 48 hpi, as determined by western blotting. (F) Schematic of regulation of iron transport and storage expression by iron regulatory proteins (IRPs). (G,H) Protein expression of IRPs in infected JEG-3 cells, as determined by western blotting. (I) Impact of excess iron supplementation (FAC) on viral titers in JEG-3 cells, as determined via plaque assay. (J-N) Protein expression of ferroptosis markers and inhibitors in SARS-CoV-2-infected JEG-3 cells over course of infection. Expression was measured by western blotting. (O) Cytotoxicity of SARS-CoV-2 Delta variant in JEG-3 cells as measured by LDH release over course of infection. (P) Cytotoxicity of JEG-3 cells treated with increasing concentrations of the FSP1 inhibitor, iFSP1, with or without infection. Cytotoxicity was determined via LDH assay. (Q) Cytotoxicity of small molecules iFSP1 and RSL3 in JEG-3 cells with or without SARS-CoV-2 infection, as measured by LDH release. (R) Viral titers in supernatants from JEG-3 cells infected with SARS-CoV-2 for 48 hours, following pre-treatment and post-inoculation exposure to iFPS1 and RSL3. Viral titers were determined via plaque assay. Data represents average of n=3 independent replicates per group ± SEM. Significance determined by Student’s unpaired t-test except for P and Q which were determined by two-way ANOVA and R which was determined by one-way ANOVA. For all tests, *p<0.05, **p<0.01, ***p<0.001, ****p<<0.001.
Article Snippet: The human trophoblast cell line JEG-3 (ATCC HTB-36) was cultured in DMEM/F-12 (Gibco, 11330032) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, 16140071).
Techniques: Variant Assay, Infection, Plaque Assay, Expressing, Western Blot, Lactate Dehydrogenase Assay
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